Michal Bilek's side complicated their World Cup hopes after drawing against Bulgaria and losing to Denmark in their last two home games, with a 3-0 win in Armenia in March at least keeping them third in Group B.
Friday's match in Prague is expected to go ahead despite floods that have left swathes of the country submerged and threatened parts of the capital's historic centre.
"We will try to raise the spirit of the people by beating Italy," goalkeeper Petr Cech told a Czech newspaper.
"It will not be easy but it all depends on us," Cech added. "A victory would put us back in the running for qualification for Brazil but one point would also keep us in the hunt. A loss would make things extremely complicated."
Rosicky's skills will mean nothing if the Czechs continue to struggle in front of goal with only six goals scored in their five matches so far.
"The Czechs are one of those teams you have to go out and beat because they don't give you anything," goalkeeper Gianluigi Buffon told reporters.
"You play a below-par game then you risk losing, if you play only normally then you might be able to grab a point but we want to play a great game to increase our lead at the top of the group."
