Van Gaal named his 30-man preliminary squad for the finals on Tuesday and half of those players will have the chance to shine in their attempts to make the final cut.
Five more individuals - Ajax goalkeeper Kenneth Vermeer, his club-mate Davy Klaassen, PSV winger Luciano Narsingh, Chelsea midfielder Marco van Ginkel and Twente striker Luc Castaignos - make up the rest of the 20-man party for Saturday's game at the Amsterdam Arena, though their hopes of making Brazil are already over.
Feyenoord duo Terence Kongolo and Tonny Vilhena will hope to make the senior bows at international level, as will three of the players in Ecuador's ranks.
Reinaldo Rueda's squad travelling to Netherlands is the same as his 24-man provisional World Cup selection - midfielders Carlos Gruezo and Jose Francisco Cevallos Jr will hope for a debut, as will experienced striker Armando Wila.
Twente attacker Quincy Promes was a surprise entrant in Van Gaal's World Cup squad, having only made his international debut against France in March.
He told his club's official website: "I'm back step closer to my goal - going to the World Cup.
"Right now I'm at the final 30 and I think that is an achievement in itself.
"If you see where I come from, this time last year I played in the Jupiler League (on loan at Go Ahead Eagles)."
"I have had a good week and very beautiful experience. I look forward to the next week with new experiences."
The nations have met just once previously, in a 2006 friendly that ended in a 1-0 win for the Dutch courtesy of Dirk Kuyt's goal.
Van Gaal's side begin the tournament with a repeat of 2010's final against Spain before facing Australia and Chile in a daunting Group B.
