The Catalan club's prospects of retaining the La Liga crown have been significantly damaged after a number of surprising defeats in recent weeks.
Martino's men were stunned 1-0 by struggling Real Valladolid last weekend, slumping to a loss against a side that is battling against relegation and had won just four previous league matches this campaign.
Valladolid's shock victory over the 22-time Spanish champions has led to scathing criticism of Martino, whose position has come under increasing scrutiny.
Osasuna did hold Barca to a 0-0 draw in the reverse fixture in October, but two consecutive defeats have seen them pulled back into the battle against relegation.
Javi Garcia's side are only three points clear of safety heading into the weekend's fixtures, with 11 games remaining, and could be pulled even further towards the drop with defeat at Barcelona.
However, despite Osasuna's current struggles, striker Oriol Riera, who spent six years as a youngster at Barcelona, is hopeful they can prove to be a test for their hosts.
"Playing against one of the top three sides is always very difficult, but we're going there keen to make things difficult for them," he told the club's official website.
"We will make their lives difficult. At least, that's what we’ll be trying to do."
Pique is one booking away from a ban and will miss next weekend's Clasico clash with Real should he receive a yellow card on Sunday.
