Serie A leaders Inter Milan will have to do without Maicon in Saturday's big match at third-placed Juventus after their appeal to have his two-match ban reduced was rejected on Friday.
The Brazil full-back was given the suspension for being sent off for dissent in a 3-1 win at Bologna two weeks ago.
Midfielder Felipe Melo is in the match squad for faltering Juve, who dropped eight points behind Inter with last week's 2-0 defeat at Cagliari, after a throat infection.
Second-placed AC Milan, who trail Inter by seven points, cannot use Filippo Inzaghi in Saturday's home match against Sampdoria, although the suspended striker has not been a regular starter of late anyway.
Sampdoria, whose strong early season form has deserted them recently, are hit even harder by bans, with defenders Fabrizio Cacciatore and Marco Rossi and midfielder Angelo Palombo out after last week's bad-tempered 3-0 defeat by city rivals Genoa.
A car with Palermo players Edinson Cavani and Nicolas Bertolo inside was attacked while they waited at a traffic light on Thursday, the Sicilian club said before Sunday's home match against Cagliari.
