Frenchman Le Guen omitted seasoned campaigners like defender Rigobert Song and Geremi and midfielder Alexandre Song from Cameroon's shock opening 1-0 loss to Japan in Bloemfontein on Monday.
"What we have seen in recent games, which we think is unfortunate, is that many young fresh players have been introduced into the team at the expense of experienced ones," midfielder Achille Emana told reporters on Thursday.
"They cannot withstand the sort of pressure that comes with playing in big tournaments such as the World Cup.
"That is why we are appealing to the coach to review the line-up for Saturday's game against Denmark."
Le Guen has not spoken to the media since after the match in Bloemfontein and was again conspicuous by his absence from Thursday's news conference.
"More often than not I am not fielded in my right position. I'm made to chase the ball all over the field. And in that case, how do you expect me to be effective?" he said.
