Mazzarri's men sit fourth in Serie A but managed a 2-0 win at home to Qarabag in the UEFA Europa League on Thursday.
Fiorentina were also in European action – and in Belarus – so Inter can have few excuses at Stadio Artemio Franchi.
Rodrigo Palacio and Pablo Daniel Osvaldo were left on the bench against Qarabag by Mazzarri, who felt they were both far from full fitness.
Osvaldo's absence would be a blow, with the Italian having scored an equal league-high four goals this campaign.
"We'll see how they are tomorrow," Mazzarri said.
"I'm not sure right now but we will of course evaluate who we can take to Florence."
It was a much-needed win for Inter, who suffered a shock 4-1 home loss to Cagliari last week.
"I don't want to waste too much time talking," he said.
"Even after a victory I want us to be focused on the next match straight away."
Despite a 3-0 win at Dinamo Minsk on Thursday, Fiorentina coach Vincenzo Montella was far from impressed by his team's display – particularly defensively.
