The striker picked the ideal time to strike in Thursday's encounter in Lisbon, with his powerful 84th-minute finish giving Benfica the upper hand heading into next week's second leg.
Benfica – who lost last season's final to Chelsea – took the lead in the second minute thanks to Ezequiel Garay's header.
Juventus had no shortage of motivation either, with this year's final to be held in Turin, and after a good second half, levelled proceedings when Carlos Tevez netted a well-taken finish with 17 minutes left.
The goal – Tevez's first in European competition since scoring for Manchester United in April 2009 – looked like it would give the Serie A outfit a crucial draw, but Lima had other ideas.
It took less than two minutes for Benfica to take the lead as Garay powerfully headed in Miralem Sulejmani's corner from the left.
Slack marking from Leonardo Bonucci allowed Garay a free header, with the defender's decision to push his opponent, instead of challenge him in the air, sure to be questioned by coach Antonio Conte.
Juventus – missing star midfielder Arturo Vidal to a knee injury – struggled to make inroads despite a good spell of possession, with Tevez not troubling Artur with a tame 20-yard effort.
An excellent slaloming run from Tevez on the half-hour mark was indicative of Juve's first-half struggles, as the sharp build-up play amounted to nothing, and when they did create another chance, defender Stephan Lichtsteiner was in an offside position when he wasted a free header.
Juve came close to levelling proceedings 10 minutes into the second half, with Artur doing well to tip Paul Pogba's header wide after Marchisio's clever centre.
The Serie A leaders kept pressing, as Lichtsteiner's teasing ball across the face of goal had to be well cleared by Maximiliano Pereira before they were eventually rewarded by Tevez.
Marchisio's ball sent Kwadwo Asamoah free down the left and his cut-back to Tevez allowed the forward time before shooting.
Lima responded in style for Benfica, though, with the forward hammering a first-time strike home from just inside the penalty area into the top-right corner after Ivan Cavaleiro's clever dummy.
The result will lift Benfica ahead of Sunday's crucial cup clash, while Juventus are likely to rest players in Monday's Serie A visit to Sassuolo.
