A 4-1 defeat in last week's last eight first leg was the Bundesliga club's heaviest loss in their continental history, and no side has ever progressed in a European tie after losing the home leg by more than two goals.
Dieter Hecking's men have enjoyed superb season overall, firmly establishing themselves as Bayern Munich's closest challengers in the Bundesliga while losing just seven times across all competitions in 2014-15.
Wolfsburg failed to bounce straight back from their humbling at the hands of Napoli by being held to a 1-1 draw by Schalke at home on Sunday, but goalkeeper Diego Benaglio feels they can prove that last Thursday's result was a blip at Stadio San Paolo.
"We want to prove on the European stage that what happened in the first leg was merely a negative dip in form, as far as our performance was concerned - albeit at the worst possible time in an extremely important game," he said.
While Wolfsburg - who will hope Kevin De Bruyne recovers from a bruised foot - have hit a relative mini-slump in the last week or so, Napoli have appeared revitalised since going five games without a win.
Rafael Benitez's side have scored 10 goals in winning their last three, with Sunday's 3-0 Serie A victory at Cagliari providing the best possible preparation for Wolfsburg's visit.
Napoli have won European club football's secondary competition once before - in 1989, when they were inspired by Diego Maradona.
And Benitez will hope one of Maradona's Argentine countrymen Gonzalo Higuian can continue his fine record in this season's Europa League.
