The Germans, who reached next year's tournament with 10 wins from 10 qualifiers, will have goalkeeper Manuel Neuer back in the squad as well as strikers Miroslav Klose and Marco Reus after they recovered from injury.
Coach Joachim Low will drop some of the changes he introduced for Friday's mediocre 3-3 friendly draw in Ukraine, including a three-man defence that looked more than shaky.
"This is an extremely serious game. We want to leave a sign that we can take on and beat the very best," Bierhoff told reporters ahead of the game in Hamburg.
"We want to finish the year with a victory and obviously beating the Dutch is always double the fun."
Last year's World Cup runners-up Netherlands, who enjoy a healthy and long-standing football rivalry with their neighbours, were jeered by their own fans after being held to a lacklustre 0-0 friendly draw by Switzerland on Friday.
"I had a good meeting with [Arsenal boss Arsene] Wenger and we reached agreement he would only play on Friday and after that we would send him back," Dutch coach Bert van Marwijk told reporters.
"Things look different for this match but it has only been two weeks since he broke his nose," the Dutch coach said.
