The sides go head-to-head in San Pedro Sula on Wednesday with the South Americans having won just one of their last 14 games on the road.
Manuel Plasencia will take charge of Venezuela, having been placed in interim charge of the national side following Cesar Farias' departure to coach Liga MX outfit Tijuana in December.
After a decade with the national team, the defender will retire from international football after the World Cup, meaning Wednesday's clash will be his last on home soil for Honduras.
He said: "I'm motivated, it's my last game here at home before the World Cup, my colleagues do not want me to retire, but it is a decision already made with my wife and family.
"It's my last game in Honduras with the selection.
"We do not care what is said outside, this is a test and we will take it seriously."
Honduras striker Carlo Costly, the side's second-top scorer in World Cup qualifying, is absent with an ankle injury, so the hosts' hopes will likely rest of Jerry Bengtson.
Four players could make their international debut in the fixture, including midfielder Pedro Ramirez - who has been in fine form for Venezuelan Primera Division leaders Zamora and striker Dany Cure - who plays his club football at Caracas, under Plasencia's management.
