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Rose, 24, has yet to represent his country at full international level, but was called up by Roy Hodgson last week when fellow left-back Luke Shaw was ruled out.
However, an unspecified injury has ended Rose's hopes of making his England debut this month.
A brief statement from the FA on Wednesday read: "Tottenham Hotspur defender Danny Rose has withdrawn from the England squad through injury.
"The left-back will play no further part in preparation for the forthcoming fixtures against Lithuania and Italy and has returned to his club."
