There was also an isolated incident of racist chanting directed at Cagliari player Victor Ibarbo from a small section of Lazio's notorious Curva Nord fans.
The chants were almost drowned out by whistles from the rest of the Olympic Stadium but it led to both captains being told the match could be suspended if the abuse continued.
"Lazio is always called a racist club which is not true," president Claudio Lotito told Sky Italia. "We have lots of non-white players.
"We can't control the actions of individual fans. Coming to these sorts of judgements you risk criminalising thousands of fans for the actions of a few.
"Since I became president... certain modes of behaviour that were there before are no longer there. We are tough on the fans when they behave in a way that is against the law but today I can say the majority of the fans behaved themselves," said Lotito.
Ibarbo's burst down the right ended with Radja Nainggolan forcing a wonder save from Federico Marchetti.
Six minutes before Klose's miss, Pulga was dismissed for complaining to referee Daniele Orsato about a foul given against his team.
He became the second coach to be red-carded after Catania's Rolando Maran was sent to the stands in their 0-0 draw with Torino earlier in the day.
Marco Sau gave Cagliari the lead after 62 minutes, evading two defenders before sending a low drive back across Marchetti's despairing dive.
Lazio then put the Sardinian side under increasing pressure and after Agazzi did well to save Antonio Candreva's smart 79th-minute shot, Abdoulay Konko tapped home the equaliser from the resulting corner.
"I don't know if it was a penalty. I'd hav
