Defender Schmelzer is out with an ankle problem, midfielder Gotze has a flu virus and Reus picked up an adductor muscle injury in Sunday's 3-2 victory over Bayer Leverkusen, German FA spokesman Jens Grittner told a news conference on Monday.
Grittner said another Dortmund player, midfielder Ilkay Gundogan, would make the trip despite a minor injury. He also added there were fitness doubts concerning defender Lars Bender and striker Miroslav Klose.
Bayern Munich midfielder Bastian Schweinsteiger had already been ruled out of Wednesday's game with a minor knock.
"Records are there to be broken," said manager Oliver Bierhoff. "A win would be a good morale boost for our team."
"I am happy to be part of the team again," he said. "It is a sensational experience because I know how quickly things can change."
