Juventus defender Giorgio Chiellini has been left out of the squad for the Coppa Italia semi-final second leg against Inter at San Siro through injury.
The centre-back returned after three matches out with a calf issue in Sunday's Serie A win over the same opponents, but lasted just 36 minutes before being replaced by Daniele Rugani in the 2-0 victory.
Juve have now confirmed he suffered an overload in his calf muscle, but avoided a tear so should be fit for the second leg of the Champions League last-16 tie against Bayern Munich on March 16.
Chiellini's defensive colleague Patrice Evra is also absent from the club's 21-man squad despite Massimiliano Allegri predicting he would return for the clash after personal problems kept him out of the Inter league fixture.
Juve lead the tie 3-0 on aggregate after the first leg and the winners will progress to Rome and meet AC Milan in May's final.
