Barrios will miss their Europa League match against Karapty Lviv on Thursday and the league match at Nuremberg on Sunday, the club said on Tuesday.
Argentine-born Barrios has scored eight league goals in 14 games this season, helping Dortmund top the table by seven points.
Get the best features, fun and footballing frolics straight to your inbox every week.
Thank you for signing up to Four Four Two. You will receive a verification email shortly.
There was a problem. Please refresh the page and try again.