Mark Milligan has been ruled out of the Socceroos' crucial World Cup clash against the Netherlands on Thursday morning (AEST) with a hamstring strain.
The scratching opens the door for James Holland, Massimo Luongo or Matt McKay, as possible replacements for the hardworking midfielder.
Milligan, who failed to train with his teammates, has been to the past three world cups for Australia but last week’s 2-1 loss to Chile last week was his first on-field action at the global showpiece.
The 28-year-old will miss at least the Oranje game, footballaustralia.com.au reported.
Coach Ange Postecoglou will be forced into another change with Ryan McGowan the expected straight swap for Ivan Franjic whose tournament was ended by injury.
Speaking about potential changes to his starting XI Postecoglou said: "We may have one or two.
"Part of it is the condition of the players and who's recovered well.
"We may make a one or two changes, or maybe more than that.
"We will keep our options open and will try a couple of things at training today.
"We've made a decision on which way we'll go but obviously we've got to get through today's session.
"I've kind of learnt from the past to keep your options open till game day just in case.
"We've looked at a couple of different ways but in terms of the personnel we've made a decision.
"So if we get through training today that's how we'll line up tomorrow."
The Netherlands created one of the shocks of the opening round of the tournament when they breezed past defending World Champions Spain 5-1.
But Postecoglou was tight-lipped at a pre-match media conference about any tinkering to the formation to deal with the Dutch threat.
"We know we have to be strong defensively, the Dutch are dangerous going forward as they showed against Spain," he said.
"We know that Van Persie and Robben will be a challenge but we knew that coming into the tournament.
"In saying that we also need to be attack minded, we can't just defend for 90 minutes."
Conditions will be cooler at the Estadio Beira-Rio in Porto Alegre where the two teams will face off from 2am Thursday (AEST).
Postecoglou added: "We just have to believe in what we do and tomorrow we will get a chance to find out where we are at.
"There's a lot at stake for both teams. For us if we don't get a result it's the end of our tournament."
Skipper Mile Jedinak said the Socceroos were keen to “rectify” the loss to Chile - when a slow start saw them down two goals within the first 20 minutes - and take to the game to their star-studded opponents.
"The key part in the other match was our response after the first 20 minutes - that is driving us for this game,” Jedinak said.
"The game-plan will be different to what we had with Chile.
"And with the cooler weather the tempo will be able to be sustained for longer periods, which will be an interesting proposition and one I think we'll be prepared for.”comments