Roma midfielder Daniele De Rossi suffered a calf injury during Wednesday's Champions League defeat against Real Madrid and is facing a four-week lay-off.
De Rossi made his return from a calf problem in the 2-0 loss at the Stadio Olimpico this week as he came off the bench for the final 13 minutes.
However, the 32-year-old picked up another similar injury, with Roma confirming on Friday he will be out for around a month.
"For the Giallorossi midfielder, who this season has made 25 appearances and scored three goals, the estimated prognosis for a return is around four weeks," the club said in a statement.
De Rossi looks set to miss five games, including a Serie A clash with third-placed Fiorentina and the return leg of Roma's last-16 tie with Madrid at the Santiago Bernabeu.
However, coach Luciano Spalletti will hope he can be in contention for a return when Inter visit the capital on March 19.
Get the best features, fun and footballing frolics straight to your inbox every week.
Thank you for signing up to Four Four Two. You will receive a verification email shortly.
There was a problem. Please refresh the page and try again.